Improved euthanasia criteria

Criteria of euthanasiaLiao et al.Improved criteria
WeightCachexiaLoss of 2 g in 48 h
Loss of 3 g in 7 days
Clinical deteriorationIncreased respiratory frequencyIncreased respiratory frequency
AnorexiaHunched back with tremor
Ascites/
/No spontaneous movement when nudged

Weight and clinical status should be evaluated once every 48 h. The improved criteria allow for more objective criteria for euthanasia of animals