IL-2 responsiveness in peripheral blood T cells before and after DC vaccination

Patient IDOverall survival (months)Pre-DC Vax CD3+CD8+ EC-50Post-DC Vax CD3+CD8+ EC-50Pre-DC Vax CD3+CD4+ EC50Post-DC Vax CD3+CD4+ EC-50
1-70833.83128.213.163.52813.39
34-73016.23126.266.0120.730.005
36-852>43.927.950.0174.4070.002
6-81517.31.8540.1960.0022.355
19-53934.98.22314.271.3861.178
GAA-0335.360.5218.6329.640.003
GAA-0511.7338.234.6911.435.245
GAA-0717.216.526.075.4465.237
GAA-0911.031.1922.387.27.7
AVERAGE:50.7019.159.313.90
SEM:16.906.383.101.30
T-test:0.0450.223

1The EC-50 for IL-2 responsiveness (pSTAT-5) was calculated in CD3+8+ and CD3+CD4+ peripheral blood T cells after a 20-minute stimulation with increasing dosages of IL-2, FACS analysis, and log transformation.