Complete list of 123 peripheral immune cell subsets analyzed by flow cytometry

1. Total CD4+ T cells • PD-L1+ CD4 • PD-1+ CD4 • EOMES+ CD4 • TCR+ CD4 • Tbet+ CD4 • BATF+ CD4 • CTLA-4+ CD4 • Tim-3+ CD4 • ICOS+ CD4  o PD-L1+ ICOS+ CD4  o PD-1+ ICOS+ CD4 • Total naïve (CCR7+CD45RA+) CD4  o PD-L1+ naïve CD4  o PD-1+ naïve CD4  o CTLA-4+ naïve CD4  o Tim-3+ naïve CD4 • Total central memory (CCR7+ CD45RA-) CD4  o PD-L1+ CM CD4  o PD-1+ CM CD4  o CTLA-4+ CM CD4  o Tim-3+ CM CD4 • Total effector memory (CCR7- CD45RA-) CD4  o PD-L1+ EM CD4  o PD-1+ EM CD4  o CTLA-4+ EM CD4  o Tim-3+ EM CD4 • Total EMRA (CCR7-CD45RA+) CD4  o PD-L1+ EMRA CD4  o PD-1+ EMRA CD4  o CTLA-4+ EMRA CD4  o Tim-3+ EMRA CD42. Total CD8+ T cells • PD-L1+ CD8 • PD-1+ CD8 • EOMES+ CD8 • TCR+ CD8 • Tbet+ CD8 • BATF+ CD8 • CTLA-4+ CD8 • Tim-3+ CD8 • Total naïve (CCR7+CD45RA+) CD8  o PD-L1+ naïve CD8  o PD-1+ naïve CD8  o CTLA-4+ naïve CD8  o Tim-3+ naïve CD8 • Total central memory (CCR7+CD45RA-) CD8  o PD-L1+ CM CD8  o PD-1+ CM CD8  o CTLA-4+ CM CD8  o Tim-3+ CM CD8 • Total effector memory (CCR7- CD45RA-) CD8  o PD-L1+ EM CD8  o PD-1+ EM CD8  o CTLA-4+ EM CD8  o Tim-3+ EM CD8 • Total EMRA (CCR7-CD45RA+) CD8  o PD-L1+ EMRA CD8  o PD-1+ EMRA CD8  o CTLA-4+ EMRA CD8  o Tim-3+ EMRA CD83. Total Tregs • PD-L1+ Tregs • PD-1+ Tregs • CTLA-4+ Tregs • ICOS+ Tregs • CD45RA+ Tregs • CD49d- Tregs4. Total B cells • PD-L1+ B cells • PD-1+ B cells • CTLA-4+ B cells • Tim-3+ B cells5. Total NK • PD-L1+ NK • PD-1+ NK • Tim-3+ NK • Total mature (CD16+ CD56dim) NK  o PD-L1+ mature NK  o PD-1+ mature NK  o Tim-3+ mature NK • Total functional intermediate (CD16+ CD56br) NK  o PD-L1+ functional intermediate NK  o PD-1+ functional intermediate NK  o Tim-3+ functional intermediate NK • Total immature (CD16- CD56br) NK  o PD-L1+ immature NK  o PD-1+ immature NK  o Tim-3+ immature NK • Total unconventional (CD16- CD56dim) NK  o PD-L1+ unconventional NK  o PD-1+ unconventional NK  o Tim-3+ unconventional NK6. Total NK-T • PD-L1+ NK-T • PD-1+ NK-T • Tim-3+ NK-T7. Total cDC • PD-L1+ cDC • PD-1+ cDC • CD83+ cDC • Tim-3+ cDC8. Total pDC • PD-L1+ pDC • PD-1+ pDC • CD83+ pDC • Tim-3+ pDC9. Total MDSC • PD-L1+ MDSC • PD-1+ MDSC • CD16+ MDSC • Total monocytic (CD14+ CD15-) MDSC  o PD-L1+ mMDSC  o PD-1+ mMDSC  o CD16+ mMDSC • Total granulocytic (CD14- CD15+) MDSC  o PD-L1+ gMDSC  o PD-1+ gMDSC  o CD16+ gMDSC • Total lineage negative (CD14- CD15-) MDSC  o PD-L1+ lin neg MDSC  o PD-1+ lin neg MDSC  o CD16+ lin neg MDSC

Nine classic subsets were identified as well as 114 refined subsets relating to maturation and function within the classic subsets (Lepone et al., ref. [15])

BATF basic leucine zipper transcription factor ATF-like, cDC conventional dendritic cells, CM central memory, CTLA-4 cytotoxic T lymphocyte-associated protein-4, EM effector memory, EMRA terminally differentiated effector memory, EOMES eomesodermin, gMDSC granulocytic MDSC; ICOS inducible T cell co-stimulator, lin neg MDSC lineage negative MDSC, MDSC myeloid derived suppressor cell, mMDSC monocytic MDSC, NK natural killer, pDC plasmacytoid DC, PD-1 programmed cell death protein 1, PD-L1 programmed cell death ligand-1, Tbet T box expressed in T cells, TCR T cell receptor, Tim-3 T cell immunoglobulin and mucin domain-3, Tregs regulatory T cells