Anti-CC49-ζ immune responses in patients
| Trial | Patient | TAG-72 post heat extraction (fold increase) | Anti-idiotype titer | |
|---|---|---|---|---|
| 1:100 | 1:1000 | |||
| C-9701 | 101 F | 8.1 | ++ | ++ |
| 201A | >2.6 | +++ | +++ | |
| 202B | 1.8 | +++ | +++ | |
| 206 K | 2.5 | + | + | |
| 105 J | 1.2 | + | ++ | |
| 106 M | 2.5 | + | + | |
| 204D | UND | - | - | |
| 102G | >1.8 | +++ | ++ | |
| 205E | >1.0 | ++ | ++ | |
| C-9702 | 301B | 1.3 | + | ++ |
| 303D | >1.4 | + | ++ | |
| 401A | NA | + | - | |
NA, data not available, UND TAG-72 undetectable.
+, 1.5- to 3-fold increase; ++, 3.1- to 5-fold increase; +++, > 5-fold increase
TAG-72 was detected in sera samples from IV-infused (trial C-9701) and intra-HA-infused (trial C-9702) CART72 cell patients following heat extraction of the TAG-72 to dissociate the antigen from inhibitory antibodies. Antibodies against the CC49 antibody were detected in all evaluable patients following infusion with CART72 cells comparing the RIA titer post-infusion versus pre-infusion. The detection of anti-CC49 antibodies coincided with the clearance of CART72 cells as measured by PCR