CyTOF panel for the phenotypic and functional analysis of immune cell subsets

	Metal label	Specificity	Antibody clone
1	115In	Dead cells	n/a
2	140Ce	Beads	n/a
3	141Pr	CD25	M-A251
4	142Nd	CD19	HIB19
5	143Nd	IL-10	JES3-9D7
6	144Nd	IL-4	MP4-25D2
7	145Nd	CD4	RPA-T4
8	146Nd	CD8	RPA-T8
9	147Sm	CD20	2H7
10	148Nd	CD57	HCD57
11	149Sm	CTLA-4	14D3
12	150Nd	MIP-1β	D21–1351
13	151Eu	CD107a	H4A3
14	152Sm	TNFa	Mab11
15	153Eu	CD45RA	HI100
16	154Sm	CD3	UCHT1
17	155Gd	CD28	L293
18	156Gd	CD38	HB-7
19	157Gd	HLA-DR	G46–6
20	158Gd	CD33	WM53
21	159 Tb	GMCSF	BVD2-21C11
22	160Gd	CD14	M5E2
23	161Dy	IFNγ	4S.B3
24	162Dy	CD69	FN50
25	163Dy	TCRγδ	B1
26	164Dy	IL-17	N49–853
27	165Ho	CD127	A019D5
28	166Er	IL-2	MQ1-17 h12
29	167Er	CD27	L128
30	168Er	CD154 (CD40L)	24–31
31	169Tm	CCR7	150503
32	170Er	PD1	EH12.1
33	171Yb	Granzyme B	GB11
34	172Yb	PD-L2	24F.10C12
35	173Yb	Perforin	B-D48
36	174Yb	CD16	3G8
37	175Lu	PD-L1	29E.2A3
38	176Yb	CD56	NCAM16.2
39	191Ir	DNA1	n/a
40	193Ir	DNA2	n/a

A 40-marker CyTOF panel for the phenotypic and functional analysis of immune cell subsets in melanoma patients treated with anti-CTLA-4 or anti-PD-1 therapy is shown. The element and isotope of the metal tag conjugated to each antibody and non-protein subject (italic) is indicated under the ‘Metal Label’ column. ‘Specificity’ indicates the target recognized by the metal-conjugated antibody or non-protein subject. 193Ir/195Ir DNA Intercalator and 115In Maleimide DOTA live/dead stain facilitate the identification of live intact singlets while calibration beads (140Ce) are important for data pre-processing. Antibody clones are listed when applicable