The univariate analyses and multivariate analyses for overall survival in the primary cohort

Prognostic Factor	Univariate analyses			Multivariate analyses			Score
Prognostic Factor	HR	95% CI	P	HR	95% CI	P	Score
Gender
Male	1
Female	1.222	0.555–2.703	0.621
Age (years)
< 60	1
≥ 60	1.595	0.700–3.631	0.266
History of smoking
No	1
Yes	1.717	0.728–4.049	0.217
History of drinking
No	1
Yes	1.164	0.550–2.463	0.691
AJCC TNM Stage
I	0.039	0.007–0.212	< 0.001	0.056	0.007–0.429	0.006	0
II	0.103	0.025–0.416	0.001	0.061	0.011–0.343	0.001	1
III	0.181	0.047–0.690	0.012	0.088	0.020–0.391	0.001	2
IVA				1			3
CD8
poor	1			1			2
rich	0.172	0.074–0.397	< 0.001	0.353	0.127–0.981	0.046	0
CD4
poor	1			1
rich	0.191	0.081–0.450	< 0.001	1.199	0.431–3.337	0.729
Foxp3
< 1%	1			1			2
≥ 1%	0.327	0.147–0.726	0.006	0.215	0.080–0.582	0.002	0
CD33
< 1%	1			1			0
≥ 1%	3.348	1.334–8.404	0.010	2.956	1.026–8.517	0.045	1.5
PD-1
< 1%	1
≥ 1%	0.607	0.274–1.345	0.219
PD-L1-TC
< 10%	1			1			0
≥ 10%	2.498	1.052–5.931	0.038	3.131	1.099–8.921	0.033	1.5
PD-L1-IC
< 1%	1
≥ 1%	0.858	0.376–1.954	0.715
Tim-3
< 1%	1			1
≥ 1%	0.478	0.210–1.088	0.078#	0.405	0.148–1.103	0.077
LAG3
< 1%	1
≥ 1%	0.391	0.093–1.652	0.201
OX-40
< 2%	1
≥ 2%	0.577	0.266–1.253	0.164
ICOS
< 6%	1
≥ 6%	0.539	0.243–1.194	0.128
IDO1-TC
< 1%	1
≥ 1%	1.410	0.671–2.962	0.364
IDO1-IC
< 6%	1
≥ 6%	1.515	0.718–3.198	0.276

HR hazard ratio, CI confidence interval, TC tumor cell, IC immune cell, ^#The risk factors with P value less than 0.1 in univariable analysis were selected into multivariate analysis