Primary antibody information for Multiplex IHC/IF panels

PositionAntibodyClone (host)/CompanyDilutionIncubation (min)TSA dye
Panel 1
 1PD-L1SP142 (rabbit)/Spring Bio1:80060620
 2PD-1EPR4877(2) (rabbit)/AbCam1:100030650
 3NSEBBS/NC/VI-H14(mouse)/Dako1:100060570
 4CD68PGM-1(mouse)/Dako1:50030540
 5CD84B11(mouse)/AbD1:10030520
 6DAPIPerkin Elmer Opal 7-color kit2 drops/ml5NA
Panel 2
 1FoxP3236A/E7(mouse)/abcam1:10030570
 2NSEBBS/NC/VI-H14(mouse)/Dako1:40030620
 3PD1EPR4877(2)(rabbit)/abcam1:500120650
 4CD4EP204(rabbit)/Sigma1:50120540
 5CD20L26(mouse)/Leica1:80030520
 6CD84B11(mouse)/AbD1:10030690
 7DAPIPerkin Elmer Opal 7-color kit2 drops/ml5NA