Immunohistochemical features of IL-30-silenced tumors developed in WT and IL-30KO mice
| shPIN-SC | IL-30shPIN-SC | ANOVAp valueb | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | IL-30KO | WT | IL-30KO | ||||||||||
| Microvessel densitya | 17.0 | ± | 4.2 | 15.5 | ± | 4.4 | 7.3 | ± | 4.0d | 3.0 | ± | 2.3c | < 0.0001 |
| Sca-1+ (%)a | 52.7 | ± | 9.7 | 48.8 | ± | 8.3 | 26.5 | ± | 6.6d | 20.3 | ± | 6.9d | < 0.0001 |
| Proliferation Index (%)a | 68.9 | ± | 8.4 | 65.0 | ± | 7.5 | 35.8 | ± | 6.0d | 36.5 | ± | 7.0d | < 0.0001 |
| Apoptotic Index (%)a | 5.8 | ± | 3.0 | 16.9 | ± | 4.4e,f | 10.2 | ± | 4.7 | 28.2 | ± | 5.6c | < 0.0001 |
| Caspase-3 (%)a | 2.1 | ± | 1.5 | 14.6 | ± | 2.5e,f | 3.3 | ± | 1.9 | 21.8 | ± | 5.0c | < 0.0001 |
| Perforin (%)a | 2.0 | ± | 1.9 | 12.3 | ± | 3.7e,f | 2.8 | ± | 2.1 | 30.0 | ± | 6.3c | < 0.0001 |
aMicrovessel density and the percentage of immunostained cells were assessed by light microscopy, at × 400 in an 85431.59 μm2 field, with Qwin image analysis software (version 2.7). Results are expressed as mean ± SD of CD31 positive microvessels per field (microvessel density), or mean percentage of positive cells/number of total cells (Sca-1, PCNA, TUNEL, caspase-3, perforin)
bOne-way ANOVA for comparisons between all groups
c p < 0.01 Tukey’s HSD test compared with IL-30shPIN-SC in WT mice and control tumors in WT and KO mice
d p < 0.01 Tukey’s HSD test compared with control tumors in WT and KO mice
e p < 0.01 Tukey’s HSD test compared with control tumors in WT mice
f p < 0.01 Tukey’s HSD test compared with IL-30shPIN-SC in WT mice