Biochemical and Biophysical Research Communications
Regular ArticleSite-Specific Mutagenesis of Mistletoe Lectin: The Role of RIP Activity in Apoptosis☆
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Cited by (43)
Immunostimulatory and anti-allergic potential of novel heterotrimeric lectin from seeds of Zizyphus mauritiana Lam
2021, International Journal of Biological MacromoleculesCitation Excerpt :The use of traditional Indian medicinal plants and its biomolecules like phytochemicals and lectins causes minor side effects compared to synthetic immunomodulatory agents [19]. Several plant lectins are known to exhibit potent immunomodulatory activity [20,21], such as in vitro stimulation of splenocytes [22], apoptotic activity [23] and mitogenic activity [24–25]. In addition, plant lectins induce nitric oxide (NO) production [26], increase phagocytic activity in macrophages and NK cells [27–29], stimulate antibody responses [30], induce the expression of pro-inflammatory and other multiple cytokines [31,32], anti-allergic activity [33], pro-healing activity [34], antibacterial and antifungal activity [35,36], etc.
Enhanced dendritic cell maturation by the B-chain of Korean mistletoe lectin (KML-B), a novel TLR4 agonist
2014, International ImmunopharmacologyCitation Excerpt :Mistletoe lectin (ML) is composed of A and B chains linked by a disulfide bond. ML is a type II ribosome-inactivating protein (RIP) and is composed of a catalytically active A-chain with rRNA N-glycosidase activity, and a B chain with carbohydrate-binding properties [12]. European mistletoe (Viscum album) lectin (EML) is classified according to its carbohydrate specificities as lectin I (d-galactose), lectin II (N-acetyl-galactosamine), or lectin III (d-galactose, N-acetyl-galactosamine) [13].
The preclinical and clinical activity of aviscumine: A potential anticancer drug
2011, European Journal of CancerCitation Excerpt :Preclinical studies have shown aviscumine to have antineoplastic and immunomodulatory properties both in vitro and in vivo. Aviscumine exerts significant cytotoxic activity similar to that of plant lectin, and it induces apoptosis in a low concentration range (fM to pM) and necrosis in higher concentration ranges.10,27–34 The mechanism of aviscumine-mediated cell death has been studied in multiple cell types; the rate of apoptosis was directly correlated to the A-chain activity, revealing that apoptosis is induced solely by the toxic A-chain.7,31
Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells
2009, Archives of Biochemistry and BiophysicsCitation Excerpt :Due to these aforementioned interesting findings, we can conclude that there may be a close link between the sugar-binding activity, hemagglutinating activity and antiproliferative activity of ConA. Previous studies have reported that mistletoe lectin-I, which belongs to the type II ribosome-inactivating protein (RIP II) family and is composed of a catalytically active A-chain with rRNA N-glycosidase activity and a B-chain with carbohydrate-binding properties, exerted potent cytotoxic effects on tumor cells [22]. Moreover, mistletoe lectin-I was reported to induce apoptosis through both caspase-8/FLICE independent of death receptor pathway and p53-independent pathway with ionizing radiation [23,24].
Phase I trial of r viscumin (INN: aviscumine) given subcutaneously in patients with advanced cancer: A study of the European Organisation for Research and Treatment of Cancer (EORTC protocol number 13001)
2008, European Journal of CancerCitation Excerpt :Aviscumine is a 57-kDa heterodimer consisting of a toxic A-chain, a site-specific type II ribosome-inactivating N-glycosidase1 and a carbohydrate-binding subunit B responsible for cellular uptake.2–4
Improvement of an enzyme linked lectin assay to determine recombinant mistletoe lectin I
2007, Journal of Pharmaceutical and Biomedical Analysis
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Abbreviations used: rMLA, recombinant mistletoe lectin A-chain; rMLA E166Q R169Q, recombinant mistletoe lectin A-chain with modified active site; rML, recombinant mistletoe lectin heterodimer; rML E166Q R169Q, recombinant mistletoe lectin heterodimer with modified active site; pML, plant-derived mistletoe lectin I; MOLT-4, human T-cell leukemia line (ECACC No. 85011413); RIP, ribosome inactivating protein
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