Elsevier

Genomics

Volume 69, Issue 2, 15 October 2000, Pages 271-274
Genomics

Short Communication
Genomic Organization of a New Candidate Tumor Suppressor Gene, LRP1B☆

https://doi.org/10.1006/geno.2000.6331Get rights and content
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Abstract

LRP1B is a novel candidate tumor suppressor gene that is inactivated by genetic and transcript alterations in nearly 50% of non-small-cell lung cancer cell lines. The gene-encoded protein is highly homologous to the gigantic lipoprotein receptor-related protein 1 (LRP1) that belongs to the family of low-density lipoprotein receptors. Using a combination of PCR-based genome walking and long-distance interexon PCR, we have determined the genomic organization of LRP1B and built a contiguous array of BAC clones spanning this gene. A total of 91 exons, varying in size from 77 bases (exon 87) to 1899 bases (exon 91), were identified in the more than 500-kb-long gene sequence. Comparative analysis of the genomic structures of LRP1B and the homologous LRP1 gene revealed a striking similarity in the location and sizes of their exons.

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☆

Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. AF122013 and AF283327–AF283416.

1

Current address: Lombardi Cancer Center, Georgetown University Medical Center, NRB W305, 3970 Reservoir Road, NW Washington, DC 20007.

2

Current address: Department of Medicine, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104.

3

To whom correspondence should be addressed at The University of Pennsylvania, 713A Stellar-Chance, 422 Curie Boulevard, Philadelphia, PA 19104-6100. Telephone: (215) 573-9575. Fax: (215) 573-7699. E-mail: [email protected].

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