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Macrophages pp 263–275Cite as

Cre Driver Mice Targeting Macrophages

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1784))

Abstract

The Cre/loxP system is a widely applied technology for site-specific genetic manipulation in mice. This system allows for deletion of the genes of interest in specific cells, tissues, and whole organism to generate a diversity of conditional knockout mouse strains. Additionally, the Cre/loxP system is useful for development of cell- and tissue-specific reporter mice for lineage tracing, and cell-specific conditional depletion models in mice. Recently, the Cre/loxP technique was extensively adopted to characterize the monocyte/macrophage biology in mouse models. Compared to other relatively homogenous immune cell types such as neutrophils, mast cells, and basophils, monocytes/macrophages represent a highly heterogeneous population which lack specific markers or transcriptional factors. Though great efforts have been made toward establishing macrophage-specific Cre driver mice in the past decade, all of the current available strains are not perfect with regard to their depletion efficiency and targeting specificity for endogenous macrophages. Here we overview the commonly used Cre driver mouse strains targeting macrophages and discuss their major applications and limitations.

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Acknowledgments

This work was supported by grants from the National Institutes of Health (R00 CA188093 to G.R. and P30 CA034196 to E.L.) of the United States. We apologize to our colleagues whose works are not cited in this manuscript due to space limitations.

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Authors and Affiliations

  1. The Jackson Laboratory, Bar Harbor, ME, USA

    Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li & Guangwen Ren

  2. Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME, USA

    Danielle Harmer

  3. The Key Laboratory of Experimental Teratology, Ministry of Education and Department of Molecular Medicine and Genetics, Shandong University School of Medicine, Jinan, Shandong, People’s Republic of China

    Peishan Li

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  1. Jiayuan Shi
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  2. Li Hua
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  3. Danielle Harmer
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Correspondence to Guangwen Ren .

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  1. CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France

    Germain Rousselet

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Shi, J., Hua, L., Harmer, D., Li, P., Ren, G. (2018). Cre Driver Mice Targeting Macrophages. In: Rousselet, G. (eds) Macrophages. Methods in Molecular Biology, vol 1784. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7837-3_24

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  • DOI: https://doi.org/10.1007/978-1-4939-7837-3_24

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