Summary
With the identification of tumor antigens, the host immune response to various types of cancers can now be studied with a high degree of specificity in large cohorts of patients, in the hope of correlating immunity with clinical events and defining immunotherapeutic strategies. Several antigens, such as NY-ESO-1, p53, or SOX2, have attracted attention because of their frequent spontaneous immunogenicity in cancer patients, notably in their ability to induce humoral responses. We describe in this chapter a simple implementation of a serological monitoring platform for autoantibody measurement in cancer patients, from production of recombinant antigens to ELISA testing and interpretation.
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Acknowledgments
The authors would like to thank Dr. Lin-Chi Chen and Erika Ritter for helping establish the method and Dr. Gerd Ritter for helpful discussions. The authors also acknowledge the Cancer Research Institute for its support.
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Gnjatic, S., Old, L., Chen, YT. (2009). Autoantibodies Against Cancer Antigens. In: Tainsky, M. (eds) Tumor Biomarker Discovery. Methods in Molecular Biology, vol 520. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-811-9_2
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DOI: https://doi.org/10.1007/978-1-60327-811-9_2
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