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The mouse Ly-17 locus identifies a polymorphism of the Fc receptor

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Abstract

The mouse Ly-17.2 alloantigen has recently been defined with both conventional and monoclonal antibodies; it identifies a locus, sited on chromosome 1, the products of which were considered to be specific for B cells. Using another Ly-17.2-specific monoclonal antibody (described herein), the tissue distribution of the Ly-17.2 antigen was shown to extend to a subpopulation of T lymphocytes and to neutrophils. This distribution is remarkably similar to that of the Fc receptor for immunoglobulin. Indeed, we now demonstrate that the Ly-17 locus codes for a polymorphism of the Fc receptor, a conclusion based upon (a) an identical tissue distribution of Ly-17.2 and FcR on both normal and tumor tissue; (b) specific inhibition of EA rosette formation by F(ab′)2 fragments of anti-Ly-17.2; (c) inhibition of the binding of the 2AG2 monoclonal rat antimouse Fc receptor antibody by Ly-17.2 antibody; (d) precipitation of an identical series of molecules by our Ly-17.2-specific antibody and by the recognized Fc receptor-specific antibody (2.4G2); and (e) the demonstration by coprecipitation that the Ly-17.2 specificity is present on Fc receptor molecules. The studies suggest that the xenogeneic monoclonal antibody (2.4G2) which recognizes an invariant site on the FcR molecule and the polymorphic site are closely associated. In addition, the studies firmly map a gene coding for or regulating the expression of the FcR to chromosome 1.

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Abbreviations

Ig:

immunoglobulin

FcR:

receptor for the Fc portion of Ig

TNP:

trinitrophenyl

Fab:

antigen-binding fragment

pA:

Protein A

SDS-PAGE:

sodium dodecyl sulfatepolyacrylamide gel electrophoresis

PBS:

phosphate-buffered saline

BSA:

bovine serum albumin

SAMIg:

sheep antimouse Ig

SRBC:

sheep red blood cells

C′:

complement

FITC:

fluorescein isothiocyanate

CNBr:

cyanogen bromide

EA:

antibody-sensitized erythrocytes

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Authors and Affiliations

  1. Research Centre for Cancer and Transplantation, Department of Pathology, University of Melbourne, 3052, Parkville, Victoria, Australia

    Margaret L. Hibbs, P. Mark Hogarth & Ian F. C. McKenzie

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  1. Margaret L. Hibbs
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  2. P. Mark Hogarth
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  3. Ian F. C. McKenzie
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Hibbs, M.L., Hogarth, P.M. & McKenzie, I.F.C. The mouse Ly-17 locus identifies a polymorphism of the Fc receptor. Immunogenetics 22, 335–348 (1985). https://doi.org/10.1007/BF00430917

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  • DOI: https://doi.org/10.1007/BF00430917

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