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Efficient generation of monoclonal antibodies from preselected antigenspecific B cells

Efficient immortalization of preselected B cells

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Abbreviations

AC:

Alternating Current

BSA:

Bovine Serum Albumin

CS:

Calf Serum

DC:

Direct Current

EBV:

Epstein-Barr Virus

ELISA:

Enzyme-linked Immunosorbent Assay

FCS:

Fetal Calf Serum

Ig:

Immunoglobulin(s)

Il-6:

Interleukin-6

MAb:

Monoclonal Antibodies

MNC:

Mononuclear Cells

PBS:

Phosphate-buffered Saline

PEG:

Poly-ethyleen-glycol

PHA:

Phytohaemagglutinin

PMA:

4-β-phorbol-12-myristate-13-acetate

T24CM:

T24 Conditioned Medium

TSN:

Human T-cell/macrophage Supernatant

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Steenbakkers, P.G.A., Hubers, H.A.J.M. & Rijnders, A.W.M. Efficient generation of monoclonal antibodies from preselected antigenspecific B cells. Mol Biol Rep 19, 125–134 (1994). https://doi.org/10.1007/BF00997158

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  • DOI: https://doi.org/10.1007/BF00997158

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