Abstract
The human epidermal growth factor receptor 2 (HER2) has been targeted as a breast cancer-associated antigen by immunotherapeutical approaches based on HER2-directed monoclonal antibodies and cancer vaccines. We describe the adoptive transfer of autologous HER2-specific T-lymphocyte clones to a patient with metastatic HER2-overexpressing breast cancer. The HLA/multimer-based monitoring of the transferred T lymphocytes revealed that the T cells rapidly disappeared from the peripheral blood. The imaging studies indicated that the T cells accumulated in the bone marrow (BM) and migrated to the liver, but were unable to penetrate into the solid metastases. The disseminated tumor cells in the BM disappeared after the completion of adoptive T-cell therapy. This study suggests the therapeutic potential for HER2-specific T cells for eliminating disseminated HER2-positive tumor cells and proposes the combination of T cell-based therapies with strategies targeting the tumor stroma to improve T-cell infiltration into solid tumors.
Abbreviations
- HER2:
-
Human epidermal growth factor receptor 2
- 111In:
-
Indium-111
- SPECT:
-
Single photon emission computed tomography
- MRI:
-
Magnetic resonance tomography
- FDG-PET:
-
[18F] Fluorodeoxyglucose positron emission tomography
- MNC:
-
Mononuclear cell
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Acknowledgments
We thank the patients for taking part in this clinical trial; Burkhard Schmidt, Evelyn Schulz and Matthias Schiemann for excellent technical assistance; Peter Schmidkonz for expert clinical care; and Wendy Batten for helpful discussion and critical reading of the manuscript. This work was supported by the Research Council of Germany grant SFB 456 (to H.B. and D.H.B.) and the Wilhelm Sander-Stiftung grant 2000.017.3 (to H.B.).
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This manuscript is published with a commentary by Vy Phan and Mary L. Disis entitled “Tumor stromal barriers to the success of adoptive T cell therapy”.
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Bernhard, H., Neudorfer, J., Gebhard, K. et al. Adoptive transfer of autologous, HER2-specific, cytotoxic T lymphocytes for the treatment of HER2-overexpressing breast cancer. Cancer Immunol Immunother 57, 271–280 (2008). https://doi.org/10.1007/s00262-007-0355-7
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DOI: https://doi.org/10.1007/s00262-007-0355-7