Abstract
Purpose
Aberrant tumor cell B7-H1 expression, a member of B7 family that can predominantly stimulate interleukin 10 (IL-10) products, contributed to the tumor immune evasion and tumor progression. This study was designed to investigate the expression of B7-H1 and IL-10 in normal pancreas tissues and pancreatic carcinoma samples, and to evaluate clinical significance of B7-H1 expression in pancreatic carcinoma.
Methods
First, the B7-H1 and IL-10 expression in 40 pancreatic carcinoma samples and 8 healthy pancreas specimens using reverse transcription-PCR (RT-PCR) and western-blotting was detected. Localization of B7-H1 and IL-10 was confirmed by immunohistochemical (IHC) staining. Next, the association between B7-H1 expression and tumor differentiation and tumor stage was analyzed. Finally, the correlation between tumor-associated B7-H1 and IL-10 was evaluated.
Results
Pancreatic carcinoma samples demonstrated the up-regulated expression of B7-H1 and IL-10 at mRNA and protein level compared with normal pancreas tissues. IHC staining revealed that B7-H1 and IL-10 was almost localized in tumor cells. Analysis of relationship between B7-H1 and tumor clinicopathological characteristics showed that B7-H1 expression was significantly associated with poor tumor differentiation (P < 0.01) and advanced tumor stage (P < 0.01). Meanwhile, tumor-associated B7-H1 expression was also correlated with IL-10 products (P < 0.01, R 2 = 0.6985, mRNA level; P < 0.01, R 2 = 0.7236, protein level) in tumor cells.
Conclusions
Our findings for the first time demonstrated up-regulated B7-H1 expression in human pancreatic carcinoma tissues, which might play a role in tumor progression and invasiveness. This expression seemed to be related to the ability of B7-H1 to promoting IL-10 secretion.
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Abbreviations
- BSA:
-
Bovine serum albumin
- IL-10:
-
Interleukin-10
- IHC:
-
Immunohistochemical
- MDC:
-
Myeloid dendritic cells
- PD-1:
-
Programmed death 1
- RT-PCR:
-
Reverse transcription-PCR
- TNM:
-
Tumor node metastasis
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Acknowledgments
This work was supported by grants from the National Key Basic Research Program of China (No.2003CB515501) and Science and Technology Department of Zhejiang Province (No. 2006C33027).
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Lei Geng and Dongsheng Huang contributed equally to this work.
Junwei Liu and Yigang Qian contributed equally to this work.
Junfang Deng and Donglin Li contributed equally to this work.
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Geng, L., Huang, D., Liu, J. et al. B7-H1 up-regulated expression in human pancreatic carcinoma tissue associates with tumor progression. J Cancer Res Clin Oncol 134, 1021–1027 (2008). https://doi.org/10.1007/s00432-008-0364-8
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DOI: https://doi.org/10.1007/s00432-008-0364-8