Abstract
The efficient isolation and ex vivo expansion of antigen-specific T cells are crucial for successful adoptive immunotherapy against uncontrollable infections and cancers. Several methods have been reported for this purpose, for example, employing MHC-multimeric complexes, interferon-gamma secretion, and antibodies specific for molecules expressed on T-cell surfaces, including CD25, CD69, CD107a, CD137, and CD154. Of the latter, CD137 has been shown to be one of the most promising targets since it is only expressed on CD8+ T cells early after encountering antigen, while being almost undetectable on resting cells. However, detailed comparisons between CD137-based and other methods have not yet been conducted. In this study, we therefore compared three approaches (with CD137, CD107a, and tetramers) using HLA-A24-restricted CMV pp65 and EBV BRLF1 epitopes as model antigens. We found that the CD137-based isolation of antigen-stimulated CD8+ T cells was comparable to tetramer-based sorting in terms of purity and superior to the other two methods in terms of subsequent cell expansion. The method was less applicable to CD4+ T cells since their CD137 upregulation is not sufficiently high. Collectively, this approach is most likely to be optimal among the methods tested for the isolation and expansion of antigen-specific CD8+ cells.
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Acknowledgments
The authors thank Dr. Hiroki Torikai, Dr. Satoko Morishima, Dr. Hidemasa Miyauchi, Dr. Ayako Demachi-Okamura, Ms. Yumi Nakao-Ohashi, Ms. Hiromi Tamaki, and Ms. Keiko Nishida for their expert technical assistance. This study was supported in part by Scientific Research on Priority Areas (B01) (no. 17016089), from the Ministry of Education, Culture, Science, Sports, and Technology, Japan; Research on Human Genome, Tissue Engineering Food Biotechnology and the Second and Third Team Comprehensive 10-year Strategy for Cancer Control (no. 26), from the Ministry of Health, Labour; and Collaboration with Medical Biological Laboratories Co., Ltd.
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K.W. and S.T. are employees of Medical Biological Laboratories Co., Ltd. S.S. is a representative executive of T Cell Technologies, Inc. Y.A. has received financial support through collaboration with Medical Biological Laboratories Co., Ltd.
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Watanabe, K., Suzuki, S., Kamei, M. et al. CD137-guided isolation and expansion of antigen-specific CD8 cells for potential use in adoptive immunotherapy. Int J Hematol 88, 311–320 (2008). https://doi.org/10.1007/s12185-008-0134-z
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DOI: https://doi.org/10.1007/s12185-008-0134-z