Gas chromatography of neutral and amino sugars in glycoproteins

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Abstract

A gas chromatographic method for determining the neutral and amino sugars commonly found in glycoproteins of animal origin is described. Following mild acid hydrolysis, the solution is neutralized with Dowex 1 HCO3, and the sugars are reduced to alditols in the cold with sodium borohydride. The solution is lyophilized, and the alditols are acetylated with acetic anhydride in pyridine. Monosaccharides can be determined on as little as 1 mg of a glycoprotein which contains 6% total carbohydrate.

References (12)

  • J.R. Clamp et al.

    Biochim. Biophys. Acta

    (1967)
  • W.F. Lehnhardt et al.

    J. Chromatog

    (1968)
  • E.F. Hartree

    Anal. Biochem

    (1964)
  • C.C. Sweeley et al.

    Anal. Chem

    (1963)
  • E.P. Crowell et al.

    Anal. Chem

    (1967)
  • J.C. Bennett

    Biochemistry

    (1968)
There are more references available in the full text version of this article.

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These studies were supported by grants from the U. S. Public Health Service, National Institute of Arthritis and Metabolic Diseases (NIAMD 03555), and the John A. Hartford Foundation.

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