Alimentary TractDimeric sialyl-Lex expression in gastric carcinoma correlates with venous invasion and poor outcome☆,☆☆
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Materials and methods
Surgical specimens from 97 gastric carcinomas resected consecutively at Hospital S. João-Medical Faculty of Porto were included in the study. All tissue samples were collected after informed consent. Tissue fragments were fixed in 10% formaldehyde and embedded in paraffin. Sections of 4 μm were cut from each representative paraffin block and stained with H&E to evaluate and classify the lesions. Serial sections of each case were used for orcein staining (detection of vascular invasion) and
Relationship between dimeric S-Lex expression and clinicopathologic features
Immunoreactivity was either absent or restricted to mucous neck cells in normal-appearing gastric mucosa distant from tumors. In the gastric mucosa at the immediate periphery of tumors, weak expression of dimeric S-Lex antigen was observed in 78% of the cases, usually in the cytoplasm of neck and foveolar/superficial cells. Dimeric S-Lex was expressed in 45 of 97 gastric carcinoma cases (46.4%). Immunoreactivity was observed at the cell membrane (Figure 1) or in the cytoplasm (Figure 2) and
Discussion
In the present study, we analyzed the immunohistochemical expression of dimeric S-Lex in gastric carcinomas and its relationship with the clinicopathologic features of the tumors and the survival rate of the patients.
In nonneoplastic mucosa, the expression of dimeric S-Lex, when present, was only observed in the neck cells. This finding is in agreement with the previously reported restricted immunoreactivity of FH6 antibody in normal adult tissues.25 In gastric carcinomas, we detected dimeric
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Address requests for reprints to: Margarida Amado, Ph.D., Institute of Molecular Pathology and Immunology of University of Porto, IPATIMUP, Rua Dr. Roberto Frias s/n, 4200 Porto, Portugal. Fax: (351) 2-557-0799.
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Supported by project 2/2.1/SAU/1356/95 from PRAXIS XXI.