Cell
Volume 169, Issue 4, 4 May 2017, Pages 750-765.e17
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Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses

https://doi.org/10.1016/j.cell.2017.04.014Get rights and content
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Highlights

  • Paired analysis reveals a unique tumor-immune signature independent of TNM stage

  • Depletion of CD141+ DC and enrichment of PPARγhi macrophages in early stage lung cancer

  • Reduced and impaired NK cells in early stage lung cancer

  • Harnessing TIM compartment may help enhance T cell immunotherapies

Summary

To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell analysis of the tumor, non-involved lung, and blood cells, we provide a detailed immune cell atlas of early lung tumors. We show that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments. Moreover, we identified changes in tumor-infiltrating myeloid cell (TIM) subsets that likely compromise anti-tumor T cell immunity. Paired single-cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies.

Keywords

human non-small cell lung cancer (NSCLC)
lung adenocarcinoma
CD141+ DC
CD1c+ DC
tumor macrophage
T Cell
NK Cell
TLS
TIM
immune cell atlas

Cited by (0)

13

Present address: Innate Pharma, 117 Avenue de Luminy, 13009 Marseille, France

14

These authors contributed equally

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