Cell Reports
Volume 20, Issue 6, 8 August 2017, Pages 1476-1489
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Cell-Type-Specific Gene Programs of the Normal Human Nephron Define Kidney Cancer Subtypes

https://doi.org/10.1016/j.celrep.2017.07.043Get rights and content
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Highlights

  • In-depth analysis of normal kidney tissue transcriptomes from the TCGA

  • Segment-specific gene signatures can be extracted from kidney tissue samples

  • The ontogeny of RCC is revealed by segment-specific gene signatures

Summary

Comprehensive transcriptome studies of cancers often rely on corresponding normal tissue samples to serve as a transcriptional reference. In this study, we performed in-depth analyses of normal kidney tissue transcriptomes from the TCGA and demonstrate that the histological variability in cellularity, inherent in the kidney architecture, lead to considerable transcriptional differences between samples. This should be considered when comparing expression profiles of normal and cancerous kidney tissues. We exploited these differences to define renal-cell-specific gene signatures and used these as a framework to analyze renal cell carcinoma (RCC) ontogeny. Chromophobe RCCs express FOXI1-driven genes that define collecting duct intercalated cells, whereas HNF-regulated genes, specific for proximal tubule cells, are an integral part of clear cell and papillary RCC transcriptomes. These networks may be used as a framework for understanding the interplay between genomic changes in RCC subtypes and the lineage-defining regulatory machinery of their non-neoplastic counterparts.

Keywords

kidney
nephron
gene expression
renal cell carcinoma
RCC
cell of origin
HIF
NHF
FOXI1

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