Basic-alimentary tractInterleukin-13 Is the Key Effector Th2 Cytokine in Ulcerative Colitis That Affects Epithelial Tight Junctions, Apoptosis, and Cell Restitution
Section snippets
Lamina Propria Mononuclear Cell Cytokine Production
Lamina propria mononuclear cells (LPMCs) were isolated from surgical specimens of patients undergoing colectomy as described previously.2 Six patients had chronic active UC, and 5 patients had CD; 4 patients without intestinal inflammation with colonic adenocarcinoma served as noninflammatory controls. The institutional review boards approved the collection of surgical specimens. Because repetitive culture of cells from the same surgical specimen showed almost identical cytokine production, we
LPMCs From Patients With UC Produce IL-13
To quantify cytokine production from inflammatory T cells, LPMCs were isolated from intestinal specimens of patients with UC and respective controls (obtained at surgery) and stimulated in culture with antibodies to CD2 and CD28. After 3 days of stimulation, cytokine release in the supernatant was measured by enzyme-linked immunosorbent assay. Cells from patients with UC produced significantly higher levels of IL-13 (985 ± 273 pg/mL) than cells from patients with CD or noninflammatory controls
Discussion
UC is associated with an epithelial barrier defect characterized by reduced active absorption and increased mucosal leakiness13, 14; these abnormalities, rather than active secretion, are responsible for the diarrhea seen in patients with UC. However, the pathologic mechanisms leading to this barrier defect are still far from clear.
One potential factor leading to the observed changes in barrier function is cytokines secreted from lymphocytes infiltrating the lamia propria. In patients with CD,
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Supported by grants from Deutsche Forschungsgemeinschaft (DFG Schu 559/7-3 and Fr 652/4-2) and the Else Kröner-Fresenius-Stiftung.