Molecular Cell
Volume 39, Issue 1, 9 July 2010, Pages 133-144
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Article
Secreted Monocytic miR-150 Enhances Targeted Endothelial Cell Migration

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Summary

MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate target gene expression at the posttranscriptional level. Here, we report that secreted miRNAs can serve as signaling molecules mediating intercellular communication. In human blood cells and cultured THP-1 cells, miR-150 was selectively packaged into microvesicles (MVs) and actively secreted. THP-1-derived MVs can enter and deliver miR-150 into human HMEC-1 cells, and elevated exogenous miR-150 effectively reduced c-Myb expression and enhanced cell migration in HMEC-1 cells. In vivo studies confirmed that intravenous injection of THP-1 MVs significantly increased the level of miR-150 in mouse blood vessels. MVs isolated from the plasma of patients with atherosclerosis contained higher levels of miR-150, and they more effectively promoted HMEC-1 cell migration than MVs from healthy donors. These results demonstrate that cells can secrete miRNAs and deliver them into recipient cells where the exogenous miRNAs can regulate target gene expression and recipient cell function.

Highlights

► MVs derived from plasma and cultured cell medium are carriers of secreted miRNAs ► Cells selectively secrete miRNAs via MVs in response to stimuli ► Secreted miRNAs serve as signaling molecules mediating intercellular communication ► Secreted monocytic miR-150 modulates targeted endothelial cell function

CELLBIO
RNA
HUMDISEASE

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These authors contributed equally to this work