Elsevier

Genomics

Volume 92, Issue 2, August 2008, Pages 122-128
Genomics

Comparison of normalization methods with microRNA microarray

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Abstract

MicroRNAs (miRNAs) are a group of RNAs that play important roles in regulating gene expression and protein translation. In a previous study, we established an oligonucleotide microarray platform to detect miRNA expression. Because it contained only hundreds of probes, data normalization was difficult. In this study, the microarray data for eight miRNAs extracted from inflamed rat dorsal root ganglion (DRG) tissue were normalized using 15 methods and compared with the results of real-time polymerase chain reaction. It was found that the miRNA microarray data normalized by the print-tip loess method were the most consistent with results from real-time polymerase chain reaction. Moreover, the same pattern was also observed in 14 different types of rat tissue. This study compares a variety of normalization methods and will be helpful in the preprocessing of miRNA microarray data.

Abbreviations

miRNA
microRNA
RT-PCR
reverse transcription polymerase chain reaction
DRG
dorsal root ganglion
CFA
complete Freund's adjuvant.

Keywords

microRNA microarray
Normalization
Print-tip loess

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1

Y.-J.H., K.T., and Z.-Y.T. contributed equally to this work.