Abstract
ANTIGENS of the HLA-A, -B, -C and -DR series have been defined mainly using sera from multiparous women1, which generally are comparatively weak, serologically complex and available in only limited quantities. Furthermore, large screening programmes are required to find usable sera, especially for rare specificities. Although antisera to HLA determinants can be produced in high titres by immunising animals of various species with purified HLA-A, -B, -C, -D antigens2–5, they usually require extensive absorption to reveal polymorphic specificity and so have not so far been used as routine tissue-typing reagents. The development of techniques for producing hybrid cell lines secreting monoclonal antibodies of defined specificity6,7, provides a way of circumventing the problems of generating human polymorphic antisera by heteroimmunisation and has already been used to produce a monoclonal antibody against the human blood group A determinant8. Here we describe the production of a cell line secreting an anti-HLA-A2 antibody.
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References
Bodmer, W. F., Batchelor, J. R., Bodmer, J. G., Festenstein, H. & Morris, P. J. Histocompatibility Testing 1977 (Munksgaard, Copenhagen, 1978).
Metzgar, R. S. & Miller, J. L. Transplantation 13, 467–471 (1972).
Sanderson, A. R. & Welsh, K. I. Transplantation 16, 304–312 (1973).
Robb, R. J. et al. Transplantation 19, 445–447 (1975).
Barnstable, C. J. thesis, Univ. Oxford (1978).
Köhler, G. & Milstein, C. Nature 256, 495–497 (1975).
Köhler, G. & Milstein, C. Eur. J. Immun. 6, 511–519 (1976).
Barnstable, C. J. et al. Cell 14, 809–820 (1978).
Parham, P. et al. J. biol. chem. 252, 7555–7562 (1977).
Williams, A. F. Contemp. Top. molec. Immun. 6, 83–116 (1977).
Arce-Gomez, B., Jones, E. A., Barnstable, C. J., Solomon, E. & Bodmer, W. F. Tissue Antigens 11, 96–112 (1978).
Köhler, G., Howe, S. C. & Milstein, C. Eur. J. Immun. 6, 292–295 (1976).
Trucco, M. M., Stocker, J. W. & Ceppellini, R. Nature 273, 666–668 (1978).
Kissmeyer-Nielsen, F., Svejgaard, A. & Hauge, M. Nature 219, 1116–1119 (1968).
Cotton, R. G. H., Secher, D. S. & Milstein, C. Eur. J. Immun. 3, 136–140 (1973).
Köhler, G., Hengartner, H. & Shulman, M. J. Eur. J. Immun. 8, 82–88 (1978).
Dick, H. M. et al. Histocompatibility Testing 1975 671–676 (Munksgaard, Copenhagen, 1975).
Williams, A. F., Galfre, G. & Milstein, C. Cell 12, 663–673 (1977).
Lampson, L. A. et al. Nature 271, 461–462 (1978).
Bodmer, W. F. Nature 237, 139–145 (1972).
Parham, P. et al. Proc. natn. Acad. Sci. U.S.A. 72, 1594–1598 (1975).
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PARHAM, P., BODMER, W. Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2. Nature 276, 397–399 (1978). https://doi.org/10.1038/276397a0
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DOI: https://doi.org/10.1038/276397a0
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