Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Transfer of specificity by murine α and β T-cell receptor genes

Abstract

T-cell receptor α- and β-chain genes were isolated from a class I major histocompatibility complex-restricted cytotoxic T-cell clone and transferred by protoplast fusion into another cytolytic T-cell clone of different specificity. Expression of the transfected α- and β genes endowed the recipient cell with the specificity of the donor cell.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Schwartz, R. H. A. Rev. Immun. 3, 237–261 (1985).

    Article  CAS  Google Scholar 

  2. Hannum, C., Freed, J. H., Tarr, G., Kappler, J. & Marrack, P. Immun. Rev. 81, 161–176 (1984).

    Article  CAS  Google Scholar 

  3. Meuer, S. C., Acuto, O., Hercend, T., Schlossman, S. F. & Reinherz, E. A. Rev. Immun. 2, 23–50 (1984).

    Article  CAS  Google Scholar 

  4. Pessano, S., Oettgen, H., Bhan, A. K. & Terhorst, C. EMBO J. 4, 337–344 (1985).

    Article  CAS  Google Scholar 

  5. Allison, J. P. & Lanier, L. L. Nature 314, 107–109 (1985).

    Article  ADS  CAS  Google Scholar 

  6. MacDonald, H. R., Glasebrook, A. L., Bron, C., Kelso, A. & Cerottini, J. C. Immun. Rev. 68, 89–115 (1982).

    Article  CAS  Google Scholar 

  7. Marrack, P. et al. J. exp. Med. 158, 1077–1091 (1983).

    Article  CAS  Google Scholar 

  8. Greenstein, J. L., Malissen, B. & Burakoff, S. J. J. exp. Med. 162, 369–374 (1985).

    Article  CAS  Google Scholar 

  9. Davis, M. M. A. Rev. Immun. 3, 537–560 (1985).

    Article  CAS  Google Scholar 

  10. Mak, T. W. & Yanagi, Y. Immun. Rev. 81, 221–233 (1984).

    Article  CAS  Google Scholar 

  11. Robertson, M. Nature 317, 768–771 (1985).

    Article  ADS  CAS  Google Scholar 

  12. Chien, Y., Gascoigne, N. R. J., Kavaler, J., Lee, N. E. & Davis, M. M. Nature 309, 322–326 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Gascoigne, N. R. J., Chien, Y., Becker, D. M., Kavaler, J. & Davis, M. M. Nature 310, 387–391 (1984).

    Article  ADS  CAS  Google Scholar 

  14. Malissen, M. et al. Cell 37, 1101–1110 (1984).

    Article  CAS  Google Scholar 

  15. Patten, P. et al. Nature 312, 40–46 (1984).

    Article  ADS  CAS  Google Scholar 

  16. Saito, H. et al. Nature 312, 36–40 (1984).

    Article  ADS  CAS  Google Scholar 

  17. Chien, Y. et al. Nature 312, 31–35 (1984).

    Article  ADS  CAS  Google Scholar 

  18. Barth, R. K. et al. Nature 316, 517–523 (1985).

    Article  ADS  CAS  Google Scholar 

  19. Behlke, M. A. et al. Science 229, 566–570 (1985).

    Article  ADS  CAS  Google Scholar 

  20. Arden, B., Klotz, J. L., Siu, G. & Hood, L. E. Nature 316, 783–787 (1985).

    Article  ADS  CAS  Google Scholar 

  21. Hayday, A. C. et al. Nature 316, 828–832 (1985).

    Article  ADS  CAS  Google Scholar 

  22. Winoto, A., Mjolsness, S. & Hood, L. Nature 316, 832–836 (1985).

    Article  ADS  CAS  Google Scholar 

  23. Becker, D. M. et al. Nature 317, 430–434 (1985).

    Article  ADS  CAS  Google Scholar 

  24. Saito, H. et al. Nature 309, 757–762 (1984).

    Article  ADS  CAS  Google Scholar 

  25. Hayday, A. C. et al. Cell 40, 259–269 (1985).

    Article  CAS  Google Scholar 

  26. Heilig, J. S. et al. Nature 317, 68–70 (1985).

    Article  ADS  CAS  Google Scholar 

  27. Ohashi, P. S. et al. Nature 316, 606–609 (1985).

    Article  ADS  CAS  Google Scholar 

  28. Yagüe, J. et al. Cell 42, 81–87 (1985).

    Article  Google Scholar 

  29. Dembić, Z., Bannwarth, W., Taylor, B. A. & Steinmetz, M. Nature 314, 271–273 (1985).

    Article  ADS  Google Scholar 

  30. Snodgrass, H. R., Dembić, Z., Steinmetz, M. & von Boehmer, H. Nature 315, 232–233 (1985).

    Article  ADS  CAS  Google Scholar 

  31. Acuto, O. et al. J. exp. Med. 161, 1326–1343 (1985).

    Article  CAS  Google Scholar 

  32. Rupp, F., Acha-Orkea, H., Hengartner, H., Zinkernagel, R. & Joho, R. Nature 315, 425–427 (1985).

    Article  ADS  CAS  Google Scholar 

  33. Gillies, S. D., Folson, V. & Tonegawa, S. Cell 33, 717–728 (1983).

    Article  CAS  Google Scholar 

  34. Picard, D. & Schaffner, W. Nature 307, 80–82 (1984).

    Article  ADS  CAS  Google Scholar 

  35. Bergman, Y., Rice, D., Grosschedl, R. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 81, 7041–7045 (1984).

    Article  ADS  CAS  Google Scholar 

  36. Haas, W. & Kisielow, P. Eur. J. Immun. 15, 755–760 (1985).

    Article  CAS  Google Scholar 

  37. Silva, A., MacDonald, H. R., Conzelmann, A., Corthésy, T. & Nabholz, M. Immun. Rev. 76, 105–129 (1983).

    Article  CAS  Google Scholar 

  38. Zamoyska, R., Vollmer, A. C., Sizer, K. C., Liaw, C. W. & Parnes, J. R. Cell 43, 153–163 (1985).

    Article  CAS  Google Scholar 

  39. Chirgwin, J., Przybyla, A., MacDonald, R. & Rutter, W. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  Google Scholar 

  40. Glisin, V., Crkvenjakov, R. & Byus, C. Biochemistry 13, 2633–2637 (1977).

    Article  Google Scholar 

  41. Feinberg, A. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).

    Article  CAS  Google Scholar 

  42. Goridis, C. et al. EMBO J. 4, 631–635 (1985).

    Article  CAS  Google Scholar 

  43. Steinmetz, M. et al. EMBO J. 3, 2995–3003 (1984).

    Article  CAS  Google Scholar 

  44. Steinmetz, M. et al. Nature 300, 35–42 (1982).

    Article  ADS  CAS  Google Scholar 

  45. Steinmetz, M., Stephan, D., Dastoornikoo, G. R., Gibb, E. & Romaniuk, R. in Immunological Methods Vol. 3 (eds Lefkovits, I. & Pernis, B.) 1–19 (Academic, New York, 1985).

    Book  Google Scholar 

  46. Frischauf, A. M., Garoff, H. & Lehrach, H. Nucleic Acids Res. 8, 5541–5549 (1980).

    Article  CAS  Google Scholar 

  47. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  48. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  49. Kiefer, H. R. & Bannwarth, W. in Immunological Methods Vol. 3 (eds Lefkovits, I. & Pernis, B.) 69–83 (Academic, New York, 1985).

    Book  Google Scholar 

  50. Grosveld, F. G. et al. Nucleic Acids Res. 21, 6715–6732 (1982).

    Article  Google Scholar 

  51. Ochi, A., Hawley, R. G., Sculman, M. J. & Hozumi, N. Nature 302, 340–342 (1983).

    Article  ADS  CAS  Google Scholar 

  52. McCubrey, J., McKearn, J. P. & Köhler, G. Eur. J. Immun. 15, 1117–1124 (1985).

    Article  CAS  Google Scholar 

  53. Zinn, K., D'Maio, D. & Maniatis, T. Cell 34, 865–879 (1983).

    Article  CAS  Google Scholar 

  54. Haas, W., Mathur-Rochat, J., Pohlit, H., Nabholz, M. & von Boehmer, H. Eur. J. Immun. 10, 828–834 (1980).

    Article  CAS  Google Scholar 

  55. Haas, W. & Kisielow, P. Eur. j. Immun. 15, 751–755 (1985).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

DembiĆ, Z., Haas, W., Weiss, S. et al. Transfer of specificity by murine α and β T-cell receptor genes. Nature 320, 232–238 (1986). https://doi.org/10.1038/320232a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/320232a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing