Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain

Nature volume 323, pages 455–458 (1986)Cite this article

Abstract

Gelsolin is representative of a class of actin-modulating proteins found in lower eukaryotes to mammals, which sever actin filaments1. Gelsolin found in the cytoplasm of cells is functionally similar to a mammalian plasma protein of similar size, originally called ADF or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25-amino-acid residue extension on plasma gelsolin which appears to account for the difference in relative molecular mass (Mr) between the proteins as assessed by SDS-polyacrylamide gel electrophoresis (PAGE), 93,000 (93K) and 90K, respectively2. Here we report the isolation of full-length human plasma gelsolin complementary DNA clones from a HepG2 library. The inferred amino-acid sequence reveals the presence of a signal peptide, a long tandem repeat that matches the actin-binding domains of gelsolin, a tetrapeptide present in actin and extended regions of identical sequence with rabbit macrophage gelsolin. Southern blot analysis indicates that a single gene in the haploid genome encodes both protein forms.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Stossel, T. P. et al. A. Rev. Cell Biol. 1, 323–402 (1985).

    Article  Google Scholar 

  2. Yin, H. L., Kwiatkowski, D. J., Mole, J. E. & Cole, F. S. J. biol. chem. 259, 5271–5276 (1984).

    CAS  PubMed  Google Scholar 

  3. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Wickner, W. T. & Lodish, H. F. Science 230, 400–407 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Yin, H. L. & Stossel, T. P. J. biol. Chem. 255, 9490–9493 (1980).

    CAS  PubMed  Google Scholar 

  6. Soua, Z., Porte, F., Harricane, M-C., Feinberg, J. & Capony, J.-P. Eur. J. Biochem. 153, 275–287 (1985).

    Article  CAS  PubMed  Google Scholar 

  7. Chaponnier, C., Janmey, P. A. & Yin, H. L. J. Cell Biol. (in the press).

  8. Bryan, J. & Hwo, S. J.Cell Biol. 102, 1439–1446 (1986).

    Article  CAS  PubMed  Google Scholar 

  9. Kwiatkowski, D. J., Janmey, P. A., Mole, J. E. & Yin, H. L. J. biol. Chem. 260, 15323–15328 (1985).

    Google Scholar 

  10. Kretsinger, R. H. CRC crit. Rev. Biochem. 8, 119–174 (1980).

    Article  CAS  PubMed  Google Scholar 

  11. Kanehisa, M. Nucleic Acids Res. 10, 183–196 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. Atlas of Protein Sequence and Structure Vol. 5 Suppl. 3, 353–358 (Natn. Biomed. Res. Fdn, Silver Spring, 1978).

    Google Scholar 

  13. Robine, S. et al. Proc. natn. Acad. Sci. U.S.A. 82, 8488–8492 (1985).

    Article  ADS  CAS  Google Scholar 

  14. Glenney, J. R. & Weber, K. Proc. natn Acad. Sci. U.S.A. 78, 2810–2814 (1981).

    Article  ADS  CAS  Google Scholar 

  15. Matsudaira, P. T. & Burgess, D. R. J. Cell Biol. 92, 648–656 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Matsudaira, P., Jakes, R. & Walker, J. E. Nature 315, 248–250 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Hesterberg, L. K. & Weber, K. J. biol. Chem. 258, 365–369 (1983).

    CAS  PubMed  Google Scholar 

  18. Sutoh, K. Biochemistry 23, 1942–1946 (1984).

    Article  CAS  PubMed  Google Scholar 

  19. Gubler, U. & Hoffman, B. J. Gene 25, 263–279 (1983).

    Article  CAS  PubMed  Google Scholar 

  20. Ginsburg, D. et al. scince 228, 1401–1406 (1985).

    Article  ADS  CAS  Google Scholar 

  21. Dale, R. M. K., McClure, B. A. & Houchins, J. P. Plasmid 13, 31–40 (1985).

    Article  CAS  PubMed  Google Scholar 

  22. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn Acad. Sci. U.S.A. 74, 5463–5469 (1977).

    Article  ADS  CAS  Google Scholar 

  23. Feinberg, A.P. & Vogelstein, B. Analyt. Biochem. 137, 266–267 (1984).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hematology-Oncology Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114, USA

    David J. Kwiatkowski, Thomas P. Stossel & Helen L. Yin

  2. Hematology Division, Department of Pediatrics, Children's Hospital, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Stuart H. Orkin

  3. Department of Biochemistry, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, USA

    John E. Mole

  4. Department of Pediatrics, St Louis Children's Hospital, Washington University School of Medicine, St Louis, Missouri, 63110, USA

    Harvey R. Coltens

Authors
  1. David J. Kwiatkowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas P. Stossel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stuart H. Orkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John E. Mole
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Harvey R. Coltens
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Helen L. Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kwiatkowski, D., Stossel, T., Orkin, S. et al. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature 323, 455–458 (1986). https://doi.org/10.1038/323455a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/323455a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing