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Phosphorylation and activation of the Jak-3 Janus kinase in response to interleukin-2

Nature volume 370, pages 151–153 (1994)Cite this article

Abstract

INTERLEUKIN-2 is an autocrine growth factor for T cells1,2 which also activates other cells including B cells3 and natural killer cells4. The subunits of the interleukin-2 receptor (IL-2R) lack intrinsic enzymatic activity, but protein tyrosine phosphorylation is a critical event following ligand binding and src family kinases, such as Lck, are known to be activated by IL-2 (refs 5–9). However, IL- 2 signalling can occur in the absence of receptor interaction with Lck, suggesting that other protein tyrosine kinases might be important10. Here we report that a new member of the Janus family of kinases (Jak-3) is coupled to the IL-2R in human peripheral blood T cells and natural killer cells.

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Authors and Affiliations

  1. Leukocyte Cell Biology Section, Laboratory of Experimental Immunology, National Cancer Institute, FCRDC, Frederick, Maryland, 21702-1201, USA

    James A. Johnston, Masaru Kawamura, John R. Ortaldo, Daniel W. McVicar & John J. O'Shea

  2. Laboratory of Molecular Immunoregulation, Biological Response Modifier Program, National Cancer Institute, FCRDC, Frederick, Maryland, 21702-1201, USA

    Robert A. Kirken

  3. Biological Carcinogenesis and Development Program, PRI/DynCorp, National Cancer Institute, FCRDC, Frederick, Maryland, 21702-1201, USA

    Yi-Qing Chen & Trevor B. Blake

  4. Advanced BioSciences Laboratories Inc., National Cancer Institute, FCRDC, Frederick, Maryland, 21702-1201, USA

    Kyoichi Shibuya

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  1. James A. Johnston
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  2. Masaru Kawamura
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  3. Robert A. Kirken
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  4. Yi-Qing Chen
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  9. John J. O'Shea
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Johnston, J., Kawamura, M., Kirken, R. et al. Phosphorylation and activation of the Jak-3 Janus kinase in response to interleukin-2. Nature 370, 151–153 (1994). https://doi.org/10.1038/370151a0

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