Abstract
A 58-mer L-RNA ligand that binds to naturally occurring D-adenosine with a dissociation constant of 1.7 μM in solution was identified from a combinatorial library employing mirror-design. The corresponding D-RNA ligand shows identical binding affinity to L-adenosine. Reciprocal chiral specificity was also evident from ligand discrimination; the binding affinity of the L-RNA ligand for D-adenosine was 9000-fold greater than its affinity for L-adenosine and vice versa. While the D-RNA ligand was rapidly degraded in human serum, the L-RNA ligand displayed an extraordinary stability. This indicates the potential application of specifically designed L-RNA ligands as stable monoclonal antibody analogues and the development of highly stable L-ribozymes.
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Klußmann, S., Nolte, A., Bald, R. et al. Mirror-image RNA that binds D-adenosine. Nat Biotechnol 14, 1112–1115 (1996). https://doi.org/10.1038/nbt0996-1112
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DOI: https://doi.org/10.1038/nbt0996-1112
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