Abstract
The nuclear receptor TLX (also known as NR2E1) is essential for adult neural stem cell self-renewal; however, the molecular mechanisms involved remain elusive. Here we show that TLX activates the canonical Wnt/β-catenin pathway in adult mouse neural stem cells. Furthermore, we demonstrate that Wnt/β-catenin signalling is important in the proliferation and self-renewal of adult neural stem cells in the presence of epidermal growth factor and fibroblast growth factor. Wnt7a and active β-catenin promote neural stem cell self-renewal, whereas the deletion of Wnt7a or the lentiviral transduction of axin, a β-catenin inhibitor, led to decreased cell proliferation in adult neurogenic areas. Lentiviral transduction of active β-catenin led to increased numbers of type B neural stem cells in the subventricular zone of adult brains, whereas deletion of Wnt7a or TLX resulted in decreased numbers of neural stem cells retaining bromodeoxyuridine label in the adult brain. Both Wnt7a and active β-catenin significantly rescued a TLX (also known as Nr2e1) short interfering RNA-induced deficiency in neural stem cell proliferation. Lentiviral transduction of an active β-catenin increased cell proliferation in neurogenic areas of TLX-null adult brains markedly. These results strongly support the hypothesis that TLX acts through the Wnt/β-catenin pathway to regulate neural stem cell proliferation and self-renewal. Moreover, this study suggests that neural stem cells can promote their own self-renewal by secreting signalling molecules that act in an autocrine/paracrine mode.
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Acknowledgements
We thank Jeremy Stark and John Zaia for a critical reading of the manuscript; Shengxiu Li and Richard Stewart for technical help; Mariko Lee and Brian Armstrong for microscopy support; Angela Barth (Stanford University) for the constitutively active β-catenin construct; Frank Costantini (Columbia University) for providing the axin cDNA; Andrew Lassar (Harvard Medical School) and Eldad Tzahor (Weizmann Institute) for providing the IgG and Frzb–IgG expression vectors and stably transfected cells; and Bert Vogelstein (Johns Hopkins University) for Topflash plasmid. Y.S. is a Kimmel Scholar. G.S. is a Herbert Horvitz Postdoctoral Fellow. F.H.G. is the Adler Professor of Age-Related Neurodegenerative Diseases and is supported by the National Institutes of Health (NIH) and the Picower Foundation. R.M.E. is an Investigator of the Howard Hughes Medical Institute at the Salk Institute and March of Dimes Chair in Molecular and Developmental Biology. This work was supported by the Whitehall Foundation, the Margaret E. Early Medical Trust and the NIH National Institute of Neurological Disorders and Stroke RO1 NS059546 (to Y.S.).
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Y.S. conceived and designed the study. Y.S., Q.Q., G.S., W.L., S.Y., P.Y. and C.Z. performed the experiments and analysed the data. Y.S., Q.Q., G.S., F.H.G. and R.M.E. interpreted the data. Y.S. wrote the paper with comments from Q.Q., G.S., R.T.Y., F.H.G. and R.M.E.
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Qu, Q., Sun, G., Li, W. et al. Orphan nuclear receptor TLX activates Wnt/β-catenin signalling to stimulate neural stem cell proliferation and self-renewal. Nat Cell Biol 12, 31–40 (2010). https://doi.org/10.1038/ncb2001
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DOI: https://doi.org/10.1038/ncb2001
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