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Chimerism

Standardisation of multiplex fluorescent short tandem repeat analysis for chimerism testing

Bone Marrow Transplantation volume 28, pages 511–518 (2001)Cite this article

Abstract

To evaluate the origin of cells after allogeneic haematopoietic stem cell transplantation we optimised and evaluated two commercially available systems (AmpFlSTR Profiler Plus and GenePrint Powerplex-16) which are based on multiplex fluorescent short tandem repeat (STR) analysis. A standard procedure for interpretation of electropherographs was found essential to obtain reproducible results. On the basis of the relative length of donor and recipient alleles, TYPE-I (no shared alleles are used to calculate chimerism), TYPE-II (one shared and one unshared allele is used to calculate chimerism) or TYPE-III (not informative) allelic distribution types were distinguished. Also, stutter peaks were recognised as an important criterion to exclude a marker for analysis. Intralaboratory and multicentre evaluation of the AmpFlSTR Profiler Plus system showed that mixed blood samples could be determined with an absolute deviation of less than 2%. A sensitivity threshold was set at 5% for TYPE-I and 10% for TYPE-II markers since relative imprecision increases at low chimerism values. No significant difference of calculated chimerism values was observed between STR markers shared between both systems. By monitoring 26 allogeneic peripheral blood stem cell transplants, the applicability of the proposed method was demonstrated. Bone Marrow Transplantation (2001) 28, 511–518.

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Acknowledgements

We thank Pascale Cochaux, Pieter De Schouwer, Dominique Latinne and Frans Houtmeyers for their contribution to the multicenter study. This study was supported by the ‘Wetenschappelijk Fonds Hematologie v.z.w., AZ Sint-Jan AV’.

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  1. Clinic and Laboratory of Haematology, AZ Sint-Jan AV, Bruges, Belgium

    F Nollet, J Billiet, D Selleslag & A Criel

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  1. F Nollet
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  2. J Billiet
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  3. D Selleslag
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  4. A Criel
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Nollet, F., Billiet, J., Selleslag, D. et al. Standardisation of multiplex fluorescent short tandem repeat analysis for chimerism testing. Bone Marrow Transplant 28, 511–518 (2001). https://doi.org/10.1038/sj.bmt.1703162

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