Basic—Alimentary TractHuman Colonic Myofibroblasts Promote Expansion of CD4+ CD25high Foxp3+ Regulatory T Cells
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Antibodies and Reagents
Please see Supplementary Materials and Methods.
Human Colonic Tissue and Primary CMF Culture
For CMF isolation, fresh human colonic mucosal sections were obtained from discarded surgical tissue in compliance with protocols approved by the University of Texas Medical Branch Institutional Review Board. Areas of uninvolved colonic tissue from patients undergoing colectomy for colon cancer were used as the source for normal CMFs (N-CMFs). CMFs were also isolated from inflamed colonic tissues from patients with ulcerative colitis (UC) or
CMFs Stabilize FoxP3 Expression in nTreg and Induce Their Proliferation in Presence of IL-2
We showed previously that CMFs induce proliferation of resting CD4+ T cells isolated from peripheral blood,12 which is also known to contain CD4+ CD25high FoxP3+nTreg cells. Thus, we investigated the interaction of the Treg and CMFs isolated from normal colonic mucosa.
Previously, we reported that, in culture, MHC class II expression by CMF drastically decreases when compared to that on acutely isolated cells and the higher levels demonstrated in situ.12 Thus, in all experiments, primary CMFs
Discussion
Antigen presenting cells play a critical role in maintaining the balance between tolerance and inflammation in the gut.1, 10, 11, 12 The role of professional antigen presenting cells, such as DCs and macrophages, in the differentiation of T cells and regulation of their activity has been well-investigated.1, 10, 11, 12, 25 Little is known about the role of intestinal stromal cells in these processes. It has been established that stromal cells are important contributors to immune homeostasis and
Acknowledgments
Drs Reyes and Powell shared senior authorship in this work.
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Conflicts of interest The authors disclose no conflicts.