Abstract
Minor or histocompatibility (H) antigens are recognized by CD4+ and CD8+ T lymphocytes as short polymorphic peptides associated with MHC molecules. They are the targets of graft versus host and graft versus leukemia responses following bone marrow transplantation between HLA-identical siblings. Several genes encoding class I-restricted minor H epitopes have been identified, but approaches used for these have proved difficult to adapt for cloning class II-restricted minor H genes. We have combined the unique antigen-presenting properties of dendritic cells and high levels of episomal expression following transfection of COS cells to identify a Y chromosome gene encoding two HY peptide epitopes, HYAb and HYEk.
MeSH terms
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Amino Acid Sequence
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Animals
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DEAD-box RNA Helicases
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism*
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Epitopes, T-Lymphocyte / biosynthesis
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Epitopes, T-Lymphocyte / genetics*
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Epitopes, T-Lymphocyte / immunology
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Eukaryotic Initiation Factor-2 / genetics
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Female
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Gene Expression Regulation / immunology
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Genes, MHC Class II*
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H-2 Antigens / genetics
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H-Y Antigen / biosynthesis
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H-Y Antigen / genetics
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Histocompatibility Antigens / biosynthesis
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Histocompatibility Antigens / genetics*
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Histocompatibility Antigens / immunology
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Histone Demethylases
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Immunity, Innate / genetics
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Mice, Transgenic
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Minor Histocompatibility Antigens / genetics
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Molecular Sequence Data
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Nuclear Proteins
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Peptides / genetics
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Peptides / immunology
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Proteins / genetics
Substances
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Epitopes, T-Lymphocyte
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Eukaryotic Initiation Factor-2
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H-2 Antigens
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H-2A antigen
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H-Y Antigen
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Histocompatibility Antigens
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Minor Histocompatibility Antigens
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Nuclear Proteins
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Peptides
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Proteins
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UTY protein, human
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Uty protein, mouse
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Histone Demethylases
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Jarid1d protein, mouse
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DDX3Y protein, human
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Ddx3y protein, mouse
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DEAD-box RNA Helicases