6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells

Immunity. 2002 Sep;17(3):289-301. doi: 10.1016/s1074-7613(02)00393-x.

Abstract

The monoclonal antibody M-DC8 defines a major subset of human blood dendritic cells (DCs). Here we identify the M-DC8 structure as 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1). In contrast to previously described blood DCs, M-DC8+ DCs lack the cutaneous lymphocyte antigen (CLA) on PSGL-1 and fail to bind P and E selectin. Yet they express anaphylatoxin receptors (C5aR and C3aR) and the Fcgamma receptor III (CD16), which recruit cells to inflammatory sites. While sharing with DC1 the expression of myeloid markers and a potent capacity to prime T cells in vitro, M-DC8+ DCs produce far more TNF-alpha in response to the bacterial endotoxin lipopolysaccharide (LPS). Thus, 6-sulfo LacNAc-expressing DCs appear as a novel proinflammatory DC subset.

MeSH terms

  • Adult
  • Amino Sugars
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, CD / analysis
  • Blood Cells / immunology
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cell Movement
  • Dendritic Cells / classification
  • Dendritic Cells / immunology*
  • Dendritic Cells / transplantation
  • E-Selectin / metabolism
  • Epitopes / chemistry*
  • Epitopes / immunology
  • Glycosylation
  • Humans
  • Inflammation / immunology
  • Lipopolysaccharides / immunology*
  • Lipopolysaccharides / pharmacology
  • Membrane Glycoproteins / chemistry*
  • Membrane Glycoproteins / immunology
  • Membrane Proteins*
  • Mice
  • Mice, SCID
  • Molecular Sequence Data
  • P-Selectin / metabolism
  • Precipitin Tests
  • Protein Processing, Post-Translational*
  • Receptor, Anaphylatoxin C5a
  • Receptors, Complement / analysis
  • Receptors, IgG / analysis
  • Transplantation, Heterologous
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • 6-sulfo-LacNac
  • Amino Sugars
  • Antibodies, Monoclonal
  • Antigens, CD
  • E-Selectin
  • Epitopes
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Membrane Proteins
  • P-Selectin
  • P-selectin ligand protein
  • Receptor, Anaphylatoxin C5a
  • Receptors, Complement
  • Receptors, IgG
  • Tumor Necrosis Factor-alpha
  • complement C3a receptor