Abstract
The transforming growth factor-β1 (TGF-β1) bioassay developed in this study monitors increased luciferase activity in MCF10A cells containing the matrix metalloproteinase-2 (MMP-2) promoter with a luciferase reporter and treated with increasing TGF-β1 concentrations. The response was linear in the concentration range from 75 to 2,500 pg/mL. The abilities of 3 types of TGF-β in inducing MMP-2 were different. The luciferase activity induced by TGF-β1 was about 2 times more than that by TGF-β2 and TGF-β3. The MMP-2 promoter bioassay showed greater reproducibility (coefficient of variation [CV] 10%) than the previously developed anticell proliferation assay of TF-1 cell (CV 16%) and the MMP-2 zymogram assay (CV 40%).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Assay*
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Cell Line
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Cloning, Molecular
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Dose-Response Relationship, Immunologic
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Enzyme Induction
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Female
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Genes, Reporter / genetics
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Humans
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Luciferases / genetics
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Luciferases / metabolism
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Luminescence*
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Mammary Glands, Human / immunology
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Mammary Glands, Human / metabolism*
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Mammary Glands, Human / pathology
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Matrix Metalloproteinase 2 / genetics*
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Matrix Metalloproteinase 2 / metabolism
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Promoter Regions, Genetic / genetics
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Reproducibility of Results
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Transforming Growth Factor beta1 / genetics
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Transforming Growth Factor beta1 / immunology
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Transforming Growth Factor beta1 / metabolism*
Substances
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Transforming Growth Factor beta1
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Luciferases
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Matrix Metalloproteinase 2