Abstract
Prostaglandin E(2) (PGE(2)) induces the expression of C-C chemokine receptor type 7 (CCR7) on human monocytes, thereby enabling their subsequent migration in response to CCL19 and CCL21, the natural ligands for CCR7. To date, important mediators of PGE(2)-mediated monocyte migration remain unknown. In this study, we explored the role of mitogen-activated protein kinases and the RhoA/Rho-associated protein kinase (ROCK) pathway in CCR7-dependent monocyte migration in the presence of PGE(2). Our results indicate that CCL19 binding to CCR7 promotes the activation of p38, extracellular signal-regulated kinase 1/2, and c-Jun N-terminal kinase and leads to monocyte migration. Moreover, the RhoA/ROCK pathway was essential for PGE(2)-mediated CCR7-dependent monocyte migration.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Line
-
Cell Movement / drug effects
-
Cell Movement / immunology
-
Chemokine CCL19 / genetics
-
Chemokine CCL19 / immunology
-
Dinoprostone / pharmacology*
-
Gene Expression Regulation / drug effects
-
Gene Expression Regulation / immunology*
-
Humans
-
JNK Mitogen-Activated Protein Kinases / genetics
-
JNK Mitogen-Activated Protein Kinases / immunology
-
Mitogen-Activated Protein Kinase 1 / genetics
-
Mitogen-Activated Protein Kinase 1 / immunology
-
Mitogen-Activated Protein Kinase 3 / genetics
-
Mitogen-Activated Protein Kinase 3 / immunology
-
Monocytes / cytology
-
Monocytes / drug effects
-
Monocytes / immunology*
-
Primary Cell Culture
-
Receptors, CCR7 / genetics*
-
Receptors, CCR7 / immunology
-
Signal Transduction / drug effects
-
Signal Transduction / genetics
-
Signal Transduction / immunology*
-
p38 Mitogen-Activated Protein Kinases / genetics
-
p38 Mitogen-Activated Protein Kinases / immunology
-
rho-Associated Kinases / genetics
-
rho-Associated Kinases / immunology
-
rhoA GTP-Binding Protein / genetics
-
rhoA GTP-Binding Protein / immunology
Substances
-
CCL19 protein, human
-
CCR7 protein, human
-
Chemokine CCL19
-
Receptors, CCR7
-
RHOA protein, human
-
rho-Associated Kinases
-
JNK Mitogen-Activated Protein Kinases
-
MAPK1 protein, human
-
Mitogen-Activated Protein Kinase 1
-
Mitogen-Activated Protein Kinase 3
-
p38 Mitogen-Activated Protein Kinases
-
rhoA GTP-Binding Protein
-
Dinoprostone