Viral acute lower respiratory infections impair CD8+ T cells through PD-1

J Clin Invest. 2012 Aug;122(8):2967-82. doi: 10.1172/JCI62860. Epub 2012 Jul 17.

Abstract

Viruses are leading causes of severe acute lower respiratory infections (LRIs). These infections evoke incomplete immunity, as individuals can be repeatedly reinfected throughout life. We report that acute viral LRI causes rapid pulmonary CD8+ cytotoxic T lymphocyte (TCD8) functional impairment via programmed death-1/programmed death ligand-1 (PD-1/PD-L1) signaling, a pathway previously associated with prolonged antigenic stimulation during chronic infections and cancer. PD-1-mediated TCD8 impairment occurred acutely in mice following infection with human metapneumovirus or influenza virus. Viral antigen was sufficient for PD-1 upregulation, but induction of PD-L1 was required for impairment. During secondary viral infection or epitope-only challenge, memory TCD8 rapidly reexpressed PD-1 and exhibited severe functional impairment. Inhibition of PD-1 signaling using monoclonal antibody blockade prevented TCD8 impairment, reduced viral titers during primary infection, and enhanced protection of immunized mice against challenge infection. Additionally, PD-1 and PD-L1 were upregulated in the lungs of patients with 2009 H1N1 influenza virus, respiratory syncytial virus, or parainfluenza virus infection. These results indicate that PD-1 mediates TCD8 functional impairment during acute viral infection and may contribute to recurrent viral LRIs. Therefore, the PD-1/PD-L1 pathway may represent a therapeutic target in the treatment of respiratory viruses.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acute Disease
  • Animals
  • Antigens, Viral
  • CD8-Positive T-Lymphocytes / immunology*
  • HLA-B7 Antigen / genetics
  • Humans
  • Imidazoles
  • Immunologic Memory
  • Influenza A Virus, H1N1 Subtype
  • Influenza, Human / immunology
  • Influenza, Human / metabolism
  • Lung / immunology
  • Lung / metabolism
  • Metapneumovirus
  • Mice
  • Mice, Transgenic
  • Paramyxoviridae Infections / immunology
  • Paramyxoviridae Infections / metabolism
  • Programmed Cell Death 1 Receptor / antagonists & inhibitors
  • Programmed Cell Death 1 Receptor / metabolism*
  • Pyridines
  • Respiratory Syncytial Virus Infections / immunology
  • Respiratory Syncytial Virus Infections / metabolism
  • Respiratory Syncytial Virus, Human
  • Respiratory Tract Infections / immunology*
  • Respiratory Tract Infections / metabolism*
  • Signal Transduction
  • Up-Regulation
  • Virus Diseases / immunology*
  • Virus Diseases / metabolism*

Substances

  • 57G709
  • Antigens, Viral
  • HLA-B*07:02 antigen
  • HLA-B7 Antigen
  • Imidazoles
  • PDCD1 protein, human
  • Pdcd1 protein, mouse
  • Programmed Cell Death 1 Receptor
  • Pyridines