Tracking down the sources of experimental contamination in microbiome studies

Genome Biol. 2014 Dec 17;15(12):564. doi: 10.1186/s13059-014-0564-2.

Abstract

A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass.

MeSH terms

  • DNA Contamination*
  • DNA, Bacterial / genetics*
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Indicators and Reagents / analysis
  • Microbiota*
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • Indicators and Reagents