Abstract
We report that oral infection with Yersinia pseudotuberculosis results in the development of two distinct populations of pathogen-specific CD8(+) tissue-resident memory T cells (TRM cells) in the lamina propria. CD103(-) T cells did not require transforming growth factor-β (TGF-β) signaling but were true resident memory cells. Unlike CD103(+)CD8(+) T cells, which were TGF-β dependent and were scattered in the tissue, CD103(-)CD8(+) T cells clustered with CD4(+) T cells and CX3CR1(+) macrophages and/or dendritic cells around areas of bacterial infection. CXCR3-dependent recruitment of cells to inflamed areas was critical for development of the CD103(-) population and pathogen clearance. Our studies have identified the 'preferential' development of CD103(-) TRM cells in inflammatory microenvironments within the lamina propria and suggest that this subset has a critical role in controlling infection.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / genetics
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Antigens, CD / immunology*
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / microbiology
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CD4-Positive T-Lymphocytes / pathology
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / microbiology
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CD8-Positive T-Lymphocytes / pathology
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Cell Movement
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Cellular Microenvironment
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Dendritic Cells / immunology
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Dendritic Cells / microbiology
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Dendritic Cells / pathology
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Gene Expression Regulation
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Immunologic Memory
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Immunophenotyping
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Integrin alpha Chains / deficiency
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Integrin alpha Chains / genetics
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Integrin alpha Chains / immunology*
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Intestinal Mucosa / immunology*
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Intestinal Mucosa / microbiology
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Intestinal Mucosa / pathology
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Macrophages / immunology
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Macrophages / microbiology
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Macrophages / pathology
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Receptors, CXCR3 / genetics
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Receptors, CXCR3 / immunology
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Signal Transduction
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / immunology
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Yersinia pseudotuberculosis / immunology
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Yersinia pseudotuberculosis Infections / genetics
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Yersinia pseudotuberculosis Infections / immunology*
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Yersinia pseudotuberculosis Infections / microbiology
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Yersinia pseudotuberculosis Infections / pathology
Substances
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Antigens, CD
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Cxcr3 protein, mouse
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Integrin alpha Chains
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Receptors, CXCR3
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Transforming Growth Factor beta
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alpha E integrins