Cassette vectors directing expression of T cell receptor genes in transgenic mice

V Kouskoff; K Signorelli; C Benoist; D Mathis

doi:10.1016/0022-1759(95)00002-r

Cassette vectors directing expression of T cell receptor genes in transgenic mice

J Immunol Methods. 1995 Mar 27;180(2):273-80. doi: 10.1016/0022-1759(95)00002-r.

Authors

V Kouskoff¹, K Signorelli, C Benoist, D Mathis

Affiliation

¹ Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, Illkirch Cedex, Strasbourg, France.

PMID: 7714342
DOI: 10.1016/0022-1759(95)00002-r

Abstract

We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Gene Expression Regulation / genetics*
Genetic Vectors / genetics*
Mice
Mice, Transgenic
Molecular Sequence Data
Mutagenesis, Insertional
Receptors, Antigen, T-Cell / biosynthesis*
Receptors, Antigen, T-Cell / genetics*

Substances

Receptors, Antigen, T-Cell