Abstract
We analyzed the expression of an IL-12 receptor by fresh dendritic cells (DC) and a DC line. Using RT-PCR, RNAse protection, and electrophoretic mobility shift assay analysis, we found that DC possess an IL-12 receptor with beta1 subunit (downstream box 1)-related differences from that on T cells. IL-12 signaling through this receptor involved members of the NF-KB but not STAT family. The unique properties of the IL-12 receptor on DC, characterized by a single class of binding sites with a Kd of about 325 pM, may underlie rather unique effects, such as IFNgamma-independent augmentation of class II antigen expression and priming for LPS-induced production of IL-12.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Nucleus / metabolism*
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Cells, Cultured
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DNA / metabolism
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / metabolism
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Dendritic Cells / drug effects*
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Dendritic Cells / metabolism*
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Dendritic Cells / ultrastructure
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Histocompatibility Antigens Class II / biosynthesis
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Histocompatibility Antigens Class II / drug effects
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Interleukin-12 / metabolism*
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Interleukin-12 / pharmacology*
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Lipopolysaccharides / pharmacology
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Male
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Mice
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Mice, Inbred DBA
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NF-kappa B / drug effects
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NF-kappa B / metabolism*
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NF-kappa B p50 Subunit
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RNA, Messenger / biosynthesis
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Receptors, Interleukin / genetics
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Receptors, Interleukin-12
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STAT3 Transcription Factor
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STAT4 Transcription Factor
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Trans-Activators / drug effects
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Trans-Activators / metabolism
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Transcription Factors / drug effects
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Transcription Factors / metabolism
Substances
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DNA-Binding Proteins
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Histocompatibility Antigens Class II
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Il12rb1 protein, mouse
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Lipopolysaccharides
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NF-kappa B
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NF-kappa B p50 Subunit
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RNA, Messenger
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Receptors, Interleukin
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Receptors, Interleukin-12
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STAT3 Transcription Factor
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STAT4 Transcription Factor
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Stat3 protein, mouse
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Stat4 protein, mouse
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Trans-Activators
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Transcription Factors
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Interleukin-12
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DNA