Coexpression of the epidermal growth factor receptor (EGFR) family receptors is found in a
subset of colon cancers, which may cooperatively promote cancer cell growth and survival …

Background The human tumor microenvironment (TME) is a complex and dynamic milieu of
diverse acellular and cellular components, creating an immunosuppressive environment …

In the third edition of this series, we described protocols for labeling cell populations with
tracking dyes, and addressed issues to be considered when combining two different tracking …

Hematopoietic stem cells are the source of all inflammatory cell types. Discovery of specific
cell surface markers unique to human hematopoietic stem (HSC) and progenitor (HSPC) cell …

PIK3CA, encoding the p110α catalytic subunit of phosphatidylinositol 3-kinase (PI3K), is
mutated in a variety of human cancers. We screened the colon cancer cell lines previously …

Acute myeloid leukemia (AML) measurable residual disease (MRD) evaluated by
multiparametric flow cytometry (MFC) is a surrogate for progression‐free and overall survival …

Exosomes, including human melanoma-derived exosomes (HMEX), are known to suppress
the function of immune effector cells, which for HMEX has been associated with the surface …

Background FoxP3 has become a key identifier of regulatory T cells. Investigators have used
a variety of antibodies and methods for detecting FoxP3 by flow cytometry. To standardize …

Background Studies evaluating peripheral patient samples show radiation can modulate
immune responses, yet the biological changes in human tumors particularly at the cellular …

Background Circulating hematopoietic progenitors (HPCs) are involved in inflammatory
diseases such as atherosclerosis, the immune response to cancer, and disorders of the …