We have genetically engineered CD4+ and CD8+ T cells with human immunodeficiency
virus (HIV) specificity by inserting a gene, CD4ζ, containing the extracellular domain of …

Background T cells engineered to express chimeric antigen receptors (CARs) have
established efficacy in the treatment of B-cell malignancies, but their relevance in solid …

During production of therapeutic monoclonal antibodies (mAb), it is highly desirable to
remove and control antibody aggregates in the manufacturing process to minimize the …

Animal cell bioreactors are often limited by the oxygen supply. The reduction in oxygen
consumption per cell that occurs under hypoxic conditions may be exploited as a method for …

We have investigated the regulation of oxygen consumption and modulation of glutathione
levels in CHO‐K1 cells under oxygen‐limiting conditions. We report here suppression of …

THIOMABs are recombinant antibodies with reactive cysteine residues used for forming
THIOMAB–drug conjugates (TDCs). We recently reported a new impurity associated with …

We have investigated the ability of anti-CD28 antibody costimulation to induce resistance to
macrophage (M)-tropic strains of human immunodeficiency virus type 1 (HIV-1) in vitro. Our …

The biopharmaceutical industry is increasing its use of the WAVE Bioreactor for culturing
cells. Although this disposable bioreactor can be equipped to provide real‐time pH and …

A microtiter-plate assay has been developed for total intracellular glutathione that facilitates
multiple-sample analysis and reduces the amount of time and chemicals required …

We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the
size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei …