The utility of ETD mass spectrometry in proteomic analysis

LM Mikesh, B Ueberheide, A Chi, JJ Coon… - … et Biophysica Acta (BBA …, 2006 - Elsevier
Mass spectrometry has played an integral role in the identification of proteins and their post-
translational modifications (PTM). However, analysis of some PTMs, such as …

Tandem mass spectrometry for peptide and protein sequence analysis

JJ Coon, JEP Syka, J Shabanowitz, DF Hunt - Biotechniques, 2005 - Future Science
Figure 1. Sequence of events defining a contemporary mass spectrometry (MS)-based
proteomics experiment.(A) The process begins with a mixture of proteins from any number of …

Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry

JEP Syka, JJ Coon, MJ Schroeder… - Proceedings of the …, 2004 - National Acad Sciences
Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem
mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an …

Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae

SB Ficarro, ML McCleland, PT Stukenberg… - Nature …, 2002 - nature.com
Protein kinases are coded by more than 2,000 genes and thus constitute the largest single
enzyme family in the human genome. Most cellular processes are in fact regulated by the …

Characterization of peptides bound to the class I MHC molecule HLA-A2. 1 by mass spectrometry

DF Hunt, RA Henderson, J Shabanowitz, K Sakaguchi… - Science, 1992 - science.org
Antigens recognized by T cells are expressed as peptides bound to major histocompatibility
complex (MHC) molecules. Microcapillary high-performance liquid chromatography …

Protein sequencing by tandem mass spectrometry.

DF Hunt, JR Yates 3rd, J Shabanowitz… - Proceedings of the …, 1986 - National Acad Sciences
Methodology for determining amino acid sequences of proteins by tandem mass
spectrometry is described. The approach involves enzymatic and/or chemical degradation of …

Characterization of histones and their post-translational modifications by mass spectrometry

BA Garcia, J Shabanowitz, DF Hunt - Current opinion in chemical biology, 2007 - Elsevier
Histone proteins and their accompanying post-translational modifications have received
much attention for their ability to affect chromatin structure and, hence, regulate gene …

Regulation of HP1–chromatin binding by histone H3 methylation and phosphorylation

W Fischle, BS Tseng, HL Dormann, BM Ueberheide… - Nature, 2005 - nature.com
Tri-methylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1
(HP1) to discrete regions of the genome, thereby regulating gene expression, chromatin …

Identification of a peptide recognized by five melanoma-specific human cytotoxic T cell lines

AL Cox, J Skipper, Y Chen, RA Henderson, TL Darrow… - Science, 1994 - science.org
Of several thousand peptides presented by the major histocompatibility molecule HLA-A2. 1,
at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem …

[HTML][HTML] Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains

JC Rice, SD Briggs, B Ueberheide, CM Barber… - Molecular cell, 2003 - cell.com
The functional significance of mono-, di-, and trimethylation of lysine residues within histone
proteins remains unclear. Antibodies developed to selectively recognize each of these …